Week Five
This week concludes the Deincoccus Caeni’s susceptibility to the tetracycline test. The concentrations used during the last experiment were 0 ug/ml, 1 ug/ml, 2 ug/ml, and 6 ug/ml. These concentrations were still too high to see any growth on the plates with antibiotics so the concentrations used were 0 ug/ml, 0.25 ug/ml, 0.5 ug/ml, and 0.75 ug/ml. Materials: Liquid culture of D. Caeni Four flasks of 25 ml TGY with Agar Tetracycline with a stock concentration of 2.5 mg/ml Hot plate Vortex Micropipette and corresponding tips Procedures: Inoculate a flask of 20 ml TGY with a colony from the D. Canei plate Place in a shaking incubator for 72 hours Autoclave four flaks of 25 ml TGY with agar to pour plates Reheat the sterilized agar on a hot plate Let cool to room temperature before adding the antibiotic Remove TGY, add tetracycline, add bacteria then vortex for six seconds on level six, pour in labeled petri plates 0 ug/ml: remove 0 ul TGY, add 250 ul of bacteria 0.25 ug/...